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Initial phase of biofouling: the microbial biofilm formation

DOI: 10.12910/EAI2014-40

Stefano Fazi, Ilaria Pizzetti, Francesca Di Pippo

The biofouling formation is a sequential process that starts with the adsorption of organic macromolecules (proteins, glycoproteins and polysaccharides). The second step, is characterized by the adhesion of prokaryotes and the subsequent development of a bacterial biofilm starting to produce a matrix of Extracellular Polymeric Substances (EPS). Here we will discuss how the bacterial community composition can be assessed during the initial phases of the biofilm development by the CAtalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD–FISH), in combination with Confocal Laser Scanning Microscopy (CLSM).
Understanding the first steps of the biofilm development process is of crucial importance for micro and macro fouling control and prevention

Stefano Fazi, Ilaria Pizzetti - Water Research Institute - National Research Council (IRSA-CNR), Monterotondo – Rome
Francesca Di Pippo

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